Mass extraction method for determining Aphelenchoides besseyi density in Oryza sativa seeds
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چکیده
منابع مشابه
A Simple Method for Determining Aphelenchoides besseyi Infestation Level of Oryza sativa Seeds.
A simple extraction method was developed for obtaining the white tip nematode, Aphelenchoides besseyi Christie, from single seeds of rice, Oryza sativa. The method was as follows: Individual rice seeds were split longitudinally and then transferred into single pipet tips. Tips containing a split seed were then singly placed upright in glass vials with water to extract the nematodes. This method...
متن کاملProtein profile of rice (Oryza sativa) seeds
Seeds are the most important plant storage organ and play a central role in the life cycle of plants. Since little is known about the protein composition of rice (Oryza sativa) seeds, in this work we used proteomic methods to obtain a reference map of rice seed proteins and identify important molecules. Overall, 480 reproducible protein spots were detected by two-dimensional electrophoresis on ...
متن کاملEffect of Water-Soaking and Air-Drying on Survival of Aphelenchoides besseyiinOryza sativa Seeds.
Several nematicides have been used to disinfest rice seeds contaminated with the white tip nematode, Aphelenchoides besseyi. We measured the efficacy of a standard disinfestation treatment that consists of soaking seeds in an aqueous emulsion of fenitrothion or fenthion and subsequently air-drying the seeds. We also measured the efficacy of the same treatments but without addition of nematicide...
متن کاملCombined mass spectrometry-based metabolite profiling of different pigmented rice (Oryza sativa L.) seeds and correlation with antioxidant activities.
Nine varieties of pigmented rice (Oryza sativa L.) seeds that were black, red, or white were used to perform metabolite profiling by using ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and gas chromatography (GC) TOF-MS, to measure antioxidant activities. Clear grouping patterns determined by the color of the rice seeds were identified in pr...
متن کاملTrehalose metabolism genes of Aphelenchoides besseyi (Nematoda: Aphelenchoididae) in hypertonic osmotic pressure survival
Some organisms can survive extreme desiccation caused by hypertonic osmotic pressure by entering a state of suspended animation known as osmobiosis. The free-living mycophagous nematode Aphelenchoides besseyi can be induced to enter osmobiosis by soaking in osmolytes. It is assumed that sugars (in particular trehalose) are instrumental for survival under environmental stress. In A. besseyi, two...
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ژورنال
عنوان ژورنال: Nematological Research (Japanese Journal of Nematology)
سال: 2002
ISSN: 0919-6765,1882-3408
DOI: 10.3725/jjn1993.32.2_25